Detection of Theileria annulata carrier cattle by PCR.
نویسندگان
چکیده
A simple method for treating bovine blood samples for direct detection of T. annulata in carriers, after polymerase chain reaction (PCR) amplification of small subunit ribosomal RNA (SSU rRNA) gene is described. The threshold of detection of the PCR-assay was an erythrocytic parasitaemia of 0.00008% corresponding to 16 infected bovine erythrocytes. In 50 known carriers, 42 were positive in PCR, in which 8 cattle revealed presence of T. annulata in stained blood smear under microscope.
منابع مشابه
Detection of Native Carrier Cattle Infected with Theileria annulata by Semi-Nested PCR and Smear Method in Golestan Province of Iran
Theileria annulata, as a pathogenic agent of tropical theileriosis, gives rise annually to serious economic losses in cattle industry of Iran. The carrier cattle, harbouring the latent forms of Theileria annulata, play a major role in infecting tick vectors and in disseminating the infection. The aim of this study was determining the carrier cattle, infected with Theileria annulata in Golestan ...
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ورودعنوان ژورنال:
- Indian journal of experimental biology
دوره 38 3 شماره
صفحات -
تاریخ انتشار 2000